Vector Cloning Services

Robust lab services to support your research needs

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Custom DNA Vector Construction Services We Provide

Porton Advanced provides custom DNA vector construction tailored to your specific research needs including, but not limited to codon sequences and regulatory elements.

We also offer a cost-effective and robust vector cloning platform with a fast turnaround time. In addition to that, Porton Advanced offers plasmid DNA preparation, virus packaging (LVV and AAV), and mRNA production.

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Services

Porton Advanced provides vector construction for shRNA vectors, gRNA vectors, Gene expression vectors, IVT vector, and more….

Workflow

Day 1 Day 2 Day 3 Day 4 Day 5 Day 6 Day 7
Preparation and handling of original vectors and fragments
Note: Gene synthesis needs additional turnaround time
Ligation and Transformation
Cultivation of Transformants
Positive clone screening and QC validation
  • 01

    Preparation of
    vector cloning

  • 02

    Ligation of linearized vector and fragment

  • 03

    Plasmid introduced into bacterial cell

  • 04

    Selection of positive cloning

  • 05

    Quality testing of vector cloning

Note: This demonstrates a regular turnaround time for vector construction if everything goes smoothly.

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Deliverables

We offer complimentary small quantities of plasmid DNAs.

Moreover, we provide a diverse range of high-quality plasmid DNA options for purchase.

Including

  • 01
    Miniprep
    >25 μg
  • 02
    Midiprep
    >350 μg
  • 03
    Maxiprep
    >1.2 mg
  • 04
    Megaprep
    >5 mg
  • 05
    Gigaprep
    >15 mg

Quality Control (QC)

Our vectors undergo rigorous quality control procedures. These include thorough DNA quantification, A260/280 measurement, electrophoresis analysis of both undigested and restriction enzyme-digested plasmid DNAs, and comprehensive Sanger sequencing.
These stringent QC measures ensure that our vectors maintain their integrity.

  • DNA Quantification
  • A260/280 Measurement
  • Electrophoresis Analysis of Both Undigested
  • Restriction Enzyme-digested Plasmid DNAs
  • Comprehensive Sanger Sequencing
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Strict Quality Assured

Clients & Partners

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