Exosome

Porton Advanced offers end-to-end exosome CDMO services, spanning early-stage research to commercial-scale production. With cutting-edge expertise in cell culture, exosome isolation, purification, and characterization, we deliver high-purity, high-quality exosome solutions. Our advanced technologies and scalable processes empower seamless transitions from R&D to commercialization, ensuring reliable and efficient outcomes for our partners.

• Cell expansion (MSC, HEK293, or other cell lines)
• GMP manufacturing (up to 500L)
• Process development and optimization
• Analytical method development and optimization, analytical qualification, and validation
• CMC information writing and regulatory submission

Process development and GMP manufacturing

Porton Advanced's technical team possesses extensive hands-on manufacturing experience in mesenchymal stem/stromal cells (MSC) and HEK293 cell culturing and expansion. Our state-of-the-art GMP facility and first-class instruments enable the reliable delivery of exosome projects.

Here is an example process for mesenchymal stem/stromal cells (MSC)/HEK293 expansion:

RCB - Shake flask/cell factory - Primary cell banking- Shake flask/cell factory - Master cell banking - Shake flask/cell factory - Working cell banking - Analytical testing- Release.

Here is an example process for exosome production:

Case studies

We achieved high-quality exosomes with a high cell density (3E7 cell/mL) after 11 days of culturing from the HEK293 cell line. 

Testing results of HEK293-based exosome samples were obtained using NanoCoulter.