Stable Cell Line Engineering

Research & Preclinical Stable Cell Line Engineering

Stable Cell Line Engineering

We offer customized editing solutions suitable for various cell scenarios, while also supporting diverse delivery methods such as RNP, lentivirus, plasmids, and multiple detection methods. This ensures efficient and stable cell lines in addition to comprehensive testing services.

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Services

01

Gene knock-out stable cell line construction
02

Gene knock-in stable cell line construction
03

Point mutation stable cell line construction

01
Gene Editing Design
- Customized editing schemes provided through multi-dimensional evaluation
- Design of sgRNA, donor DNA, and relevant vectors
02
Cell Culture and Transfection
- Applicable to various cell scenarios
- Diverse delivery methods
03
Identification and Detection of Transfected Cells
- Multi-dimensional evaluation of editing efficiency
- Multi-angle detection of cell function
04
Cell Line Delivery
- Various forms of delivery such as RNP, lentivirus, plasmids, and multiple detection methods
- Detailed delivery reports

Workflow

01

Gene Editing Design
02

Cell Culture and Transfection
03

Cell Culture and Transfection
04

Identification and Detection of Transfected Cells
05

Cell Line Delivery

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CRISPR/Cas nucleases Gene Editing System

Cas9/cas12 (knockout/knock-in), CRISPRi/a (silencing/activation), Base Editor (base editing), Primer Editor (primer editing), and Epigenetic Regulation.

Including

01

Cas9/cas12
02

CRISPRi/a
03

Base Editor
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Primer Editor
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Epigenetic Regulation

Custom Services for Varying Types of Cell Line Generation

Gene knock-out (KO) cell line generation by CRISPR

Type	Turnaround time	Price
Single KO pool	6-9 weeks	$2,999 and up
Single KO clone (frameshift)	10-14 weeks	$3,899 and up
Single KO clone (deletion)	10-14 weeks	$6,799 and up
Double KO pool（frameshift)	Please inquire	Please inquire
Double KO clone (frameshift)	Please inquire	Please inquire
Please inquire	Please inquire	Please inquire
Double KO clone (deletion)	Please inquire	Please inquire

Note: For cell pool, we deliver 2 vials with >10E6 cells/vial. For single clone, we also deliver 2 vials with >10E6 cells/vial.

Gene knock-in (KI) cell line generation by CRISPR

Type	Turnaround time	Price
Cell pool (fragment < 3kb)	8-12 weeks	$5,599
Single clone (fragment < 3kb)	14-18 weeks	$8,599
Cell pool (fragment > 3kb)	10-16 weeks	$7,999
Single clone (fragment > 3kb)	16-22 weeks	$11,599
Double KI pool	Please inquire	Please inquire
Double KI clone	Please inquire	Please inquire
Triple KI pool	Please inquire	Please inquire
Triple KI clone	Please inquire	Please inquire

Note: Typically, we provide one clone cell lines. If your project requires two clones, please contact our sales team.

Cell line generation by Base Editor, Primer Editor, CRISPR i/a, or Point Mutation

Type	Turnaround time	Price
Cell pool	8-12 weeks	$5,999 and up
Single clone	14-18 weeks	$8,599 and up

Quality Control (QC) Testing and Analysis Includes:

Verification using sanger sequencing, mycoplasma testing, and sterility testing
Analysis of off-target effects (Sanger Sequencing, NGS)
Phenotypic analysis and functional validation tests (Cell Proliferation, Apoptosis, Migration, Cell Viability, Cytotoxicity, etc.)
The following tests can be completed at additional cost as needed:
Expression tests such as RT-qPCR, WB, and FACS

Gene overexpression stable cell line (using lentivirus)

Type	Turnaround time	Price
Cell pool	6-10 weeks	$2,899 and up
Single clone	12-18 weeks	$3,899 and up

Cell line generation by Base Editor, Primer Editor, CRISPR i/a, or Point Mutation

Type	Turnaround time	Price
Cell pool)	8-12 weeks	$5,999 and up
Single clone	14-18 weeks	$8,599 and up

Note: For cell pool, we deliver 2 vials with >10E6 cells/vial.

Quality Control (QC) Testing and Analysis Includes:

Expression tests (RT-qPCR, mycoplasma testing, and sterility testing)
The following tests can be completed at additional cost as needed:
- Expression tests (WB, FACS)
- Analysis of off-target effects (Sanger Sequencing, NGS)
- Phenotypic analysis and functional validation tests (Cell Proliferation, Apoptosis, Migration, Cell Viability, Cytotoxicity, etc.)

Deliverables

Stable Cell Line

Various forms of delivery such as RNP, lentivirus, plasmids, and multiple detection methods
Detailed delivery reports

Report

Includes relevant sequences, experimental records, and detection results.

Stable Cell Line Engineering