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mRNA and LNP

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Research & Preclinical mRNA and LNP

Unlock the Potential of mRNA

mRNA therapeutics hold the potential to transform modern medicine by providing a CGT platform with the capacity for rapid development and wide-scale deployment.

Compared to recombinant proteins expressed in mammalian cell lines, manufacturing of mRNA is faster and more flexible as it can be easily produced by in vitro transcription.

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Our Custom Services

Porton Advanced provides codon optimization, custom-made mRNA, custom-made circular RNA, and premade mRNA with options: capping, modified nucleotides, or lipid nanoparticle (LNP).

Our codon optimization includes comprehensive consideration of RNA secondary structure, GC content and distribution, repeated sequences, and codon usage frequency. Our team of experts is dedicated to collaborating closely with you, ensuring that every RNA molecule we provide aligns seamlessly with your research objectives.

Our circRNA molecules are meticulously crafted to accelerate your research breakthroughs. Unlock the potential of circRNA and illuminate new pathways in your scientific inquiries.

  • 01
    Codon Optimization
  • 02
    Custom-made mRNA
  • 03
    Custom-made
    Circular RNA
  • 04
    Premade mRNA

Accelerate Your Research Breakthroughs

Our ready-to-use IVT mRNA can be used as a control for your mRNA experiment, or to assess the efficiency of mRNA delivery systems. For research-grade custom IVT mRNA preparation, please check our CRO services below. For scalable manufacturing of mRNA drugs, please check ou our CDMO services.

Products

  • 01

    Custom-made mRNA

    With Option of Capping, Modified Nucleotides, or Lipid Nanoparticles (LNPs)

    Experience the epitome of precision with our custom-made mRNA solutions. Tailor your experiments the options below, enhancing mRNA stability and translational efficiency.

    More Options

    • Advanced Capping (Cap1)
    • Modified Ncleotide Options (N1-Methylpseudouridine-m1Ψ, 5-methylcytosine-m5C, etc.)
    • Lipid Nanoparticle (LNP)
  • 02

    Custom-Made circRNA

    Delve into the world of circular RNA with our custom-made circRNA products. Circular RNAs have emerged as pivotal players in gene regulation, and our circRNA solutions are engineered to empower your investigations.

  • 03

    Pre-made mRNA

    With the option of capping, Modified Nucleotides

    Our premade mRNA (EGFP/ mCherry/ Luciferase/ Cas9) was generated with cap1 and with or without modified nucleotide, N1-Methylpseudouridine (m1Ψ). Both the protein expression (EGFP/ mCherry/ Luciferase) and the editing function (Cas9) of our pre-made mRNA have been fully validated for high levels in the cell culture.

  • IVT mRNA
  • mRNA LNP
  • mRNA tLNP
  • LNP Circular RNA (CircRNA)
  • Product Nucleotide Scale
    mRNA-eGFP
    Unmodified 100μg, 1mg
    m1Ψ 100μg, 1mg
    mRNA-Firefly Luciferase
    Unmodified 100μg, 1mg
    m1Ψ 100μg, 1mg
    mRNA-mCherry
    Unmodified 100μg, 1mg
    m1Ψ 100μg, 1mg
    mRNA-Cy5 mCherry
    Unmodified 100μg, 1mg
    m1Ψ 100μg, 1mg
    mRNA-Cas9
    Unmodified 100μg, 1mg
    m1Ψ 100μg, 1mg
    mRNA-Cre
    Unmodified 100μg, 1mg
    m1Ψ 100μg, 1mg
    mRNA-CD19 CAR
    Unmodified 100μg, 1mg, 5mg
    m1Ψ 100μg, 1mg, 5mg
  • Product Nucleotide Scale
    SM-102-LNP-eGFP
    Unmodified 100μg, 1mg
    m1Ψ 100μg, 1mg
    SM-102-LNP-Firefly Luciferase
    Unmodified 100μg, 1mg
    m1Ψ 100μg, 1mg
    SM-102-LNP-mCherry
    Unmodified 100μg, 1mg
    m1Ψ 100μg, 1mg, 5mg
    SM-102-LNP-CD19 CAR
    Unmodified 100μg, 1mg, 5mg
    m1Ψ 100μg, 1mg, 5mg
    ALC-0315-LNP-eGFP
    Unmodified 100μg, 1mg
    m1Ψ 100μg, 1mg
    ALC-0315-LNP-Firefly Luciferase
    Unmodified 100μg, 1mg
    m1Ψ 100μg, 1mg
    ALC-0315-LNP-mCherry
    Unmodified 100μg, 1mg
    m1Ψ 100μg, 1mg, 5mg
    ALC-0315-LNP-CD19 CAR
    Unmodified 100μg, 1mg, 5mg
    m1Ψ 100μg, 1mg, 5mg
  • Product Nucleotide Scale
    SM-102-0.1% Maleimide-Empty-tLNP-eGFP
    Unmodified 100μg, 1mg
    m1Ψ 100μg, 1mg
    SM-102-0.1% Maleimide-Empty-tLNP-Firefly Luciferase
    Unmodified 100μg, 1mg
    m1Ψ 100μg, 1mg
    SM-102-0.1% Maleimide-Empty-tLNP-mCherry
    Unmodified 100μg, 1mg
    m1Ψ 100μg, 1mg
    SM-102-0.1% Maleimide-Empty-tLNP-CD19 CAR
    Unmodified 100μg, 1mg, 5mg
    m1Ψ 100μg, 1mg, 5mg
    ALC-0315-0.1% Maleimide-Empty-tLNP-eGFP-100μg
    Unmodified 100μg, 1mg
    m1Ψ 100μg, 1mg
    ALC-0315-0.1% Maleimide-Empty-tLNP-Firefly Luciferase
    Unmodified 100μg, 1mg
    m1Ψ 100μg, 1mg
    ALC-0315-0.1% Maleimide-Empty-tLNP-Firefly Luciferase
    Unmodified 100μg, 1mg
    m1Ψ 100μg, 1mg
    SM-102-0.1% Maleimide-Empty-tLNP-eGFP
    Unmodified 100μg, 1mg
    m1Ψ 100μg, 1mg
  • Product Scale
    EGFP CircRNA
    50ug
    Luciferase CircRNA
    50ug

mRNA Case Studies

  • 01
    mRNA Integrity by CGE
  • 02
    mRNA Capping Ratio by LC – MS
  • 03
    mRNA-LNP Particle Size Distribution by SEC-MALS
  • 04
    LNP-circRNA transfection efficiency and biocompatibility

  • Porton Advanced’s Analytical platform utilized the CGE method for assessing mRNA integrity. Results showed a high level of mRNA intactness, meeting the process development standard.

    *Source: Porton Advanced’s Analytical Development Platform

  • Porton Advanced’s Analytical platform utilized the CGE method for assessing mRNA integrity. Results showed a high level of mRNA intactness, meeting the process development standard.

    *Source: Porton Advanced’s Analytical Development Platform

  • Porton Advanced’s Analytical platform utilized the CGE method for assessing mRNA integrity. Results showed a high level of mRNA intactness, meeting the process development standard.

    *Source: Porton Advanced’s Analytical Development Platform

  • Porton Advanced’s Analytical platform utilized the CGE method for assessing mRNA integrity. Results showed a high level of mRNA intactness, meeting the process development standard.

    *Source: Porton Advanced’s Analytical Development Platform

Workflow

  • 01

    mRNA SCDNA Design & Cloning

  • 02

    Plasmid Linearization and Purification

  • 03

    mRNA Synthesis
    by IVT

  • 04

    mRNA Synthesis
    by IVT

  • 05

    mRNA Synthesis
    by IVT

  • 06

    mRNA SCDNA Design & Cloning

  • 07

    Plasmid Linearization and Purification

  • 08

    mRNA Synthesis
    by IVT

  • 09

    mRNA Synthesis
    by IVT

  • 10

    mRNA Synthesis
    by IVT

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Quality Control (QC)

Attribute Method Research Grade
General
Appearance
Visual inspection
pH
Potentiometric analysis Optional
Identity
mRNA length
AGE
mRNA sequence
Sanger sequencing
Content
mRNA concentration
UV spectrophotometry
General
A260/A280
UV spectrophotometry
mRNA integrity
AGE
CGE
Capping efficiency
LC-MS Optional
PolyA tail length,distribution
LC-MS Optional
Impurity
Appearance
Visual inspection
pH
Potentiometric analysis Optional
Potency
Appearance
Visual inspection
pH
Potentiometric analysis Optional
Safety
Appearance
Visual inspection
pH
Potentiometric analysis Optional

Deliverables

IVT mRNA Product

Certificate of Analysis (CoA)

Storage

Our mRNA products are stored in Tris buffer (pH 7.4) and can be stored at -80°C for up to 12 months. Both products are shipped on dry ice and should not be subjected to frequent freeze-thaw cycles.

  • Storage Medium Stored in Tris buffer (pH 7.4)
  • temperature and time Can be stored at -80°C for up to 12 months
  • Shipping method Dry Ice Transport

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